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KMID : 0604520120380020103
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Journal of the Society of Cosmetic Scientists of Korea
2012 Volume.38 No. 2 p.103 ~ p.118
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Cellular Protective Effect and Liposome Formulation for Enhanced Transdermal Delivery of Isoquercitrin
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Jo Na-Rae
Gu Hyun-A
Park Su-Ah
Han Seat-Byeol
Park Soo-Nam
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Abstract
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In this study, the cellular protective effect of isoquercitrin against H2O2 and rose bengal-indued HaCaT cell damage was investigated. The ethosome and elastic liposome for enhanced transdermal delivery were prepared. Particle size, loading efficiency and cumulative permeated amounts of them were evaluated. Isoquercitrin didn't show any characteristic cytotoxicity at 50 μM. When HaCaT cells were treated with 5 mM H2O2 and 25 μM rose bengal, isoquercitrin protected the cells against the oxidative damage in a concentration dependent manner (6.25 ~ 50 μM). The size of 0.03 % isoquercitrin loaded ethosome was 222.85 nm and the loading efficiency was 82.26 %. The ethosome loaded with 0.03 % isoquercitrin was stable and maintained the constant particle size for 2 weeks after being prepared. The ethosome exhibited more enhanced skin permeability than general liposome and ethanol solution. The optimal ratio of lipid to surfactant of 0.1 % isoquercitrin loaded elastic liposomes was observed to be 89 : 5 through evaluating particle size (341.2 nm), deformability index (59.89), loading efficiency (54.3 %), and skin permeability (54.4 %).
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KEYWORD
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isoquercitrin, cellular protective effect, ethosome, elastic liposome
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